Difference between revisions of "Part:BBa K3570007"
Line 5: Line 5:
 
<h2>Usage</h2>
 
<h2>Usage</h2>
 
<p style="text-indent: 40px">
 
<p style="text-indent: 40px">
−
DPP1 downstream homologous arm part shall be used together with DPP1 downstream part ([https://parts.igem.org/Part:BBa_K3570006 BBa_K3570006]) to form a functional yeast integration locus. When DPP1 upstream put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.</p>
+
DPP1 downstream homology arm part shall be used together with DPP1 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570006 BBa_K3570006]) to form a functional yeast integration locus. When DPP1 upstream put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.</p>
  
 
<h2>Experiments</h2>
 
<h2>Experiments</h2>

Revision as of 04:43, 13 October 2020


DPP1 downstream homologous sequence

Usage

DPP1 downstream homology arm part shall be used together with DPP1 downstream homology arm part (BBa_K3570006) to form a functional yeast integration locus. When DPP1 upstream put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.

Experiments

Source

S. cerevisiae genome, chromosome IV, DPP1 gene. GenBank: CP046084.1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]