Difference between revisions of "Part:BBa K3570009"
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HO upstream homologous arm used together with HO downstream ([https://parts.igem.org/Part:BBa_K3570010 BBa_K3570010]). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
 
HO upstream homologous arm used together with HO downstream ([https://parts.igem.org/Part:BBa_K3570010 BBa_K3570010]). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
  
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<h2>Caracterisation</h2>
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<h2>Experiments</h2>
  
 
<h2>Source</h2>
 
<h2>Source</h2>

Revision as of 19:05, 12 October 2020


HO upstream homologous sequence

Usage

HO upstream homologous arm used together with HO downstream (BBa_K3570010). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.

Experiments

Source

  • [2]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]