Difference between revisions of "Part:BBa K3570006"
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DPP1 upstream homologous arm part shall be used together with DPP1 downstream part ([https://parts.igem.org/Part:BBa_K3570007 BBa_K3570007]) to form a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae's</i> genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene. </p>
 
DPP1 upstream homologous arm part shall be used together with DPP1 downstream part ([https://parts.igem.org/Part:BBa_K3570007 BBa_K3570007]) to form a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae's</i> genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene. </p>
  
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<h2>Caracterisation</h2>
+
<h2>Experiments</h2>
  
 
<h2>Source</h2>
 
<h2>Source</h2>

Revision as of 19:05, 12 October 2020


DPP1 upstream homologous sequence

Usage

DPP1 upstream homologous arm part shall be used together with DPP1 downstream part (BBa_K3570007) to form a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.

Experiments

Source

S. cerevisiae genome, chromosome IV, DPP1 gene. GenBank: CP046084.1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]