Difference between revisions of "Part:BBa K3629001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional [https://parts.igem.org/Part:BBa_K2983050 BBa_K2983050] promoter were made to remove these sites. | |
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===Source=== | ===Source=== | ||
Revision as of 02:58, 19 October 2020
Yarrowia lipolytica TEF1 promoter with an intron
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional BBa_K2983050 promoter were made to remove these sites.
Source
From the genomic sequence of wild-type Y. lipolytica strain CLIB122.