Difference between revisions of "Part:BBa K3629005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Codon optimized for expression and function in <i>Yarrowia lipolytica.</i> | + | Codon optimized for expression and function in <i>Yarrowia lipolytica.</I> The native signal peptide from <i>P. funiculosum</i> was also removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i> |
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===Source=== | ===Source=== |
Latest revision as of 02:48, 19 October 2020
Modified Penicillium funiculosum CBHI with 6X His tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 564
Illegal BamHI site found at 1048 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 46
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from P. funiculosum was also removed so it would not interfere with fused secretion tags native to Y. lipolytica
Source
Modified genomic sequence of cellobiohydrolase I from Penicillium funiculosum.