Difference between revisions of "Part:BBa K3629003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica [https://parts.igem.org/Part:BBa_K1592000 (BBa_K1592000)] | + | The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from <i>Y. lipolytica </i>[https://parts.igem.org/Part:BBa_K1592000 (BBa_K1592000)] |
===Design=== | ===Design=== | ||
Revision as of 02:10, 19 October 2020
Yarrowia lipolytica TEF1 promoter with first half of Lip2 signal peptide
TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide (BBa_K1592000)from Y. lipolytica.
Usage and Biology
The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000)
Design
Upon digestion with BbsI and this part can be assembled into a destination vector (containing BBa_K3629012) with a coding sequence that contains the last 57nts (19 codons) of the Lip2 signal peptide at the 5' end by using Gibson Assembly. In our part collection, this part can be assembled with ________ into the BBa_K3629012 destination vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 53
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]