Difference between revisions of "Part:BBa K3629019"
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<partinfo>BBa_K3629019 short</partinfo>
 
<partinfo>BBa_K3629019 short</partinfo>
  
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2-isopropylmalate (LEU4) gene from <i>Yarrowia lipolytica </i>.
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2-isopropylmalate (LEU4) coding sequence from <i>Yarrowia lipolytica </i>.
  
 
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Revision as of 07:15, 12 October 2020


Yarrowia lipolytica LEU4 gene

2-isopropylmalate (LEU4) coding sequence from Yarrowia lipolytica .

Usage and Biology

The YALI0B07447g is the gene for Yarrowia lipolytica's 2-isopropylmalate synthase which is a LEU4 or

2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme specially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 728
    Illegal BamHI site found at 293
    Illegal XhoI site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 799
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 544

References