Difference between revisions of "Part:BBa K3505006"

Revision as of 09:37, 13 October 2020

Tyr1-AIDAc Tyrosinase fused to membrane protein AIDAc. GB compatible B3-B5

Tyrosinase catalyzes the conversion of L-Tyrosine to L-Dopa and L-Dopa quinone. These derivatives can be detected electochemically, producing peak currents.[1] AIDA is native E.coli outer membrane protein. AIDA's CDS constists of 3 parts: [2] 1) A Signal Peptide that is cleaved in order the rest of the protein to be transported to the outer membrane. 2) The AIDAc is the autotransporter with the transmembrane part. 3) The passeger of AIDA which is the part that is on the exterior side.

We placed as passenger the Tyr1 gene coding to Rhizobium etli tyrosinase Because is the smallest of all tyrosinases (34kDa) and lack cysteines[3] compared to the melA tyrosinase BBa_K193600 used in iGEM before.

Sequence and Features