Difference between revisions of "Part:BBa R0011"
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The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bbpart>BBa_C0010</bbpart>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). | The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bbpart>BBa_C0010</bbpart>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Strong promoter. [jb, 5/24/04] | Strong promoter. [jb, 5/24/04] | ||
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<partinfo>BBa_R0011 SequenceAndFeatures</partinfo> | <partinfo>BBa_R0011 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_R0011 parameters</partinfo> | <partinfo>BBa_R0011 parameters</partinfo> | ||
| + | <!-- --> | ||
Revision as of 00:59, 5 June 2006
Promoter (lacI regulated, lambda pL hybrid)
Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0011, etc.)
The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0010) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
Usage and Biology
Strong promoter. [jb, 5/24/04]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]