Difference between revisions of "Part:BBa K3329021"

 
 
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This sequence code for Streptococcal Collagen-like protein, which is used to modify the bacteria cellulose.Such protein is capable of recreating the texture of leather.
 
This sequence code for Streptococcal Collagen-like protein, which is used to modify the bacteria cellulose.Such protein is capable of recreating the texture of leather.
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The wild type of this protein usually contains a V domain which is responsible for stabilizing the downstream folding process of scl2 protein. However, in our project, we designed a SpyTag003-scl2 with V domain-SpyTag003-scl2 without V domain-SpyTag003 structure, in which the V domain in first scl2 protein can stabilize the folding of the other scl2 downstream it. Therefore, the scl2 protein in our project has two different versions from the perspective of V domain. Moreover, due to the different codon preference of P. Pastoris and E. coli, the sequence of scl2 should also be divided into two different types — one for P. Pastoris, and one for E. coli. Together, considering these two factors, we generated scl2 proteins with 4 different types and 2 different versions. The first two don't contain V domain, and the second two contain V domain. Also, the first version (V1) refers to the codon optimization for P. Pastoris, and V2 refers to the codon optimization for E. coli.
  
  

Latest revision as of 09:00, 27 October 2020


Streptococcal Collagen-like 2 with V domain optimized V2

This sequence code for Streptococcal Collagen-like protein, which is used to modify the bacteria cellulose.Such protein is capable of recreating the texture of leather.

The wild type of this protein usually contains a V domain which is responsible for stabilizing the downstream folding process of scl2 protein. However, in our project, we designed a SpyTag003-scl2 with V domain-SpyTag003-scl2 without V domain-SpyTag003 structure, in which the V domain in first scl2 protein can stabilize the folding of the other scl2 downstream it. Therefore, the scl2 protein in our project has two different versions from the perspective of V domain. Moreover, due to the different codon preference of P. Pastoris and E. coli, the sequence of scl2 should also be divided into two different types — one for P. Pastoris, and one for E. coli. Together, considering these two factors, we generated scl2 proteins with 4 different types and 2 different versions. The first two don't contain V domain, and the second two contain V domain. Also, the first version (V1) refers to the codon optimization for P. Pastoris, and V2 refers to the codon optimization for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 442
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal BsaI site found at 814
    Illegal BsaI.rc site found at 439