Difference between revisions of "Part:BBa K3570013"
Line 3: Line 3:
 
<partinfo>BBa_K3570013 short</partinfo>
 
<partinfo>BBa_K3570013 short</partinfo>
  
−
The URA3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP).
 
  
−
When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired the wanted plasmid.
+
<h2>Biology</h2>
 +
<p style="text-indent: 40px">
 +
The URA3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP). </p>
 +
<p style="text-indent: 40px">
 +
When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired the wanted plasmid.</p>
  
−
Source:  
+
<h2>Experiments</h2>
 +
<p style="text-indent: 40px">
 +
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
 +
</p>
 +
 
 +
<h2>References:</h2>
 
Taken from FL38 plasmid (https://www.addgene.org/vector-database/2750/)
 
Taken from FL38 plasmid (https://www.addgene.org/vector-database/2750/)
  

Revision as of 22:43, 9 October 2020


URA3 selection marker


Biology

The URA3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP).

When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired the wanted plasmid.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References:

Taken from FL38 plasmid (https://www.addgene.org/vector-database/2750/)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
    Illegal BglII site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]