Difference between revisions of "Part:BBa K3380152"
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Malachite Green aptamer is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore. | Malachite Green aptamer is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore. | ||
| − | + | ===Characterization=== | |
| − | === | + | The Malachite Green fluorescent RNA aptamer was described by [https://pubs.acs.org/doi/full/10.1021/ja037994o Babendure, Adams and Tsien in 2003]. |
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| + | The Malachite Green aptamer folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below. | ||
| + | |||
| + | [[File:Part BBa K3380151.png|400px|DIR2s-Apt folding]] | ||
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Latest revision as of 14:19, 8 October 2020
Malachite Green fluorescent RNA aptamer
Malachite Green aptamer is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore.
Characterization
The Malachite Green fluorescent RNA aptamer was described by Babendure, Adams and Tsien in 2003.
The Malachite Green aptamer folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
Illegal BamHI site found at 33 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]