Difference between revisions of "Part:BBa K3380151"
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The DIR2s-Apt folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below. | The DIR2s-Apt folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below. | ||
| − | [[File:Part BBa K3380151.png|400px| | + | [[File:Part BBa K3380151.png|400px|DIR2s-Apt folding]] |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | It is usually used in live-cell imaging, however it can also be used in vitro. DIR2-Apt can be used for visualisation by gene fusion to the target of interest. Its fluorescent colour depends on the fluorophore it binds to. It can bind to DIR ( dimethylindole red) derivatives to give a red colour and OTB (oxazole thiazole blue) derivatives to give a blue colour. The emission spectra also depends on the bound fluorophore. The [https://pubs.acs.org/doi/full/10.1021/jacs.7b04211/ DIR-Pro]fluorophore has an excitation wavelength of 600 (nm) and an emission wavelength of 658 (nm). The [https://pubs.acs.org/doi/full/10.1021/jacs.7b04211 OTB-SO3]fluorophore has an excitation wavelength of 380 (nm) and an emission wavelength of 421 (nm). | ||
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Revision as of 13:43, 8 October 2020
DIR2s-Apt fluorescent RNA aptamer
DIR2s-Apt is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore.
Characterization
The DIR2s-Apt fluorescent RNA aptamer was described by Tan et al. (2017).
The DIR2s-Apt folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below.
Usage and Biology
It is usually used in live-cell imaging, however it can also be used in vitro. DIR2-Apt can be used for visualisation by gene fusion to the target of interest. Its fluorescent colour depends on the fluorophore it binds to. It can bind to DIR ( dimethylindole red) derivatives to give a red colour and OTB (oxazole thiazole blue) derivatives to give a blue colour. The emission spectra also depends on the bound fluorophore. The DIR-Profluorophore has an excitation wavelength of 600 (nm) and an emission wavelength of 658 (nm). The OTB-SO3fluorophore has an excitation wavelength of 380 (nm) and an emission wavelength of 421 (nm).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]