Difference between revisions of "Part:BBa K3487001"
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<partinfo>BBa_K3487001 short</partinfo> | <partinfo>BBa_K3487001 short</partinfo> | ||
| − | Description | + | ===Description=== |
| − | + | A long-chain protein formed by polymerizing four identical short TP-I monomer peptides. | |
| + | |||
| + | ===Usage and Biology=== | ||
After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides. | After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides. | ||
| + | [[File:T--SZPT-CHINA--TP-1M.png|400px|thumb|center|By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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| − | + | ||
| + | ===Characterization=== | ||
| + | By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained. | ||
| + | |||
| + | ===Sequence and Features=== | ||
| + | |||
<partinfo>BBa_K3487001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3487001 SequenceAndFeatures</partinfo> | ||
Revision as of 12:59, 18 October 2020
TP-ⅠM,A polymer of Tachyplesin Ⅰ
Description
A long-chain protein formed by polymerizing four identical short TP-I monomer peptides.
Usage and Biology
After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides.
By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.
Characterization
By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]