Difference between revisions of "Part:BBa K3519011"

 
Line 3: Line 3:
 
<partinfo>BBa_K3519011 short</partinfo>
 
<partinfo>BBa_K3519011 short</partinfo>
  
−
This part contains the RFP downstream to the arabinose inducible promoter. This has been designed keeping in mind a tightly regulated 'kill switch'. Look at the part BBa_K3519011 for the kill switch. The RFP gene here is expressed in the addition of arabinose to the media. Excess glucose in the media represses the expression of RFP.
+
This part contains the RFP downstream to the arabinose inducible promoter. This has been designed keeping in mind a tightly regulated 'kill switch'. Look at the part BBa_K3519012 for the kill switch. The RFP gene here is expressed in the addition of arabinose to the media. Excess glucose in the media represses the expression of RFP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:51, 7 October 2020


araC-araBAD promoter-mRFP

This part contains the RFP downstream to the arabinose inducible promoter. This has been designed keeping in mind a tightly regulated 'kill switch'. Look at the part BBa_K3519012 for the kill switch. The RFP gene here is expressed in the addition of arabinose to the media. Excess glucose in the media represses the expression of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
    Illegal NheI site found at 1066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1388
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1223
    Illegal AgeI site found at 2030
    Illegal AgeI site found at 2142
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1205