Difference between revisions of "Part:BBa K3519003"

 
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<partinfo>BBa_K3519003 short</partinfo>
 
<partinfo>BBa_K3519003 short</partinfo>
  
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This part codes for the gene traT. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli F factor.  
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This part codes for the gene traT. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli F factor. The simultaneous overexpression of both these genes in E. coli has been shown to reduce mating pair formation by 33,000 times. Thus, their overexpression helps reduce conjugation significantly.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 10:14, 24 October 2020


traT

This part codes for the gene traT. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli F factor. The simultaneous overexpression of both these genes in E. coli has been shown to reduce mating pair formation by 33,000 times. Thus, their overexpression helps reduce conjugation significantly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 642
    Illegal BsaI.rc site found at 124