Difference between revisions of "Part:BBa K3519002"
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<partinfo>BBa_K3519002 short</partinfo> | <partinfo>BBa_K3519002 short</partinfo> | ||
| − | This part codes for the gene traS. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli F factor. | + | This part codes for the gene traS. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli Fertiity factor (F factor). TraS is an inner membrane protein that prevents DNA transfer in stable mating pairs. The simultaneous overexpression of both these genes in E. coli has been shown to reduce mating pair formation by 33,000 times. Thus, they help reduce conjugation significantly. |
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Revision as of 10:24, 24 October 2020
traS
This part codes for the gene traS. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli Fertiity factor (F factor). TraS is an inner membrane protein that prevents DNA transfer in stable mating pairs. The simultaneous overexpression of both these genes in E. coli has been shown to reduce mating pair formation by 33,000 times. Thus, they help reduce conjugation significantly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]