Difference between revisions of "Part:BBa K3349004"

Revision as of 15:11, 7 October 2020

Pectin Lyase PelB-GFP

This Pectin Lyase is a hypothetical protein from Paenibaciullus amylolyticus. With Pnl and PelC, PelB is able to cleave and therefore breakdown pectin or homogalacturonan substrates. This protein has been characterized to cleave methylated pectin (at unmethylated sites) and polygalacturonic acid. Studies by Keggi and Doran-Peterson determined that PelB has a Km of 50.1+/- 140 mg/ml and a Vmax of 745 +/-2024 IU/mg with a 80% methylated substrate. The optimal temperature for PelB activity was determined to be 70°C [1].

For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or the basic gene part BBa_K3349001.

A GFP reporter was added to the C-terminus of the gene. As well, a C-terminal his tag was added to the GFP for nickel affinity purification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 261
Illegal BamHI site found at 1146
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3349004 short</partinfo>
-This Pectin Lyase is a hypothetical protein from Paenibaciullus amylolyticus . With Pnl and PelC, PelB is able to cleave and therefore breakdown pectin or homogalacturonan substrates. This protein has been characterized to cleave methylated pectin (at unmethylated sites) and polygalacturonic acid. Studies by Keggi and Doran-Peterson, PelB has a Km of 50.1+/- 140 mg/ml and a Vmax of 745 +/-2024 IU/mg with a 80% methylated substrate (0 and 55% methylation was also studied). The optimal temperature for PelB activity was determined to be 70°C [1].
+This Pectin Lyase is a hypothetical protein from <i>Paenibaciullus amylolyticus</i>. With Pnl and PelC, PelB is able to cleave and therefore breakdown pectin or homogalacturonan substrates. This protein has been characterized to cleave methylated pectin (at unmethylated sites) and polygalacturonic acid. Studies by Keggi and Doran-Peterson determined that PelB has a Km of 50.1+/- 140 mg/ml and a Vmax of 745 +/-2024 IU/mg with a 80% methylated substrate. The optimal temperature for PelB activity was determined to be 70°C [1].
 For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or the basic gene part BBa_K3349001.
-A GFP reporter was added to the C-terminus of the gene. As well, a C-terminal his tagwas added to the GFP for nickel affinity purification.
+A GFP reporter was added to the C-terminus of the gene. As well, a C-terminal his tag was added to the GFP for nickel affinity purification.
 <!-- Add more about the biology of this part here