Difference between revisions of "Part:BBa K3349009:Design"
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===References=== | ===References=== | ||
| − | 1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19. | + | 1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of <i>Paenibacillus amylolyticus</i> 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19. |
Latest revision as of 15:22, 7 October 2020
Pectin Lyase Pnl-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for E. coli. The his tag was placed on the C-terminus. A TEV cut site was edded between the GFP and Pectase in case cleavage is needed after pruification.
Source
The GFP protein was taken from the uniprot database (P42212). The pectin lyase was also taken from the uniprot database and the sequence ID from NCBI is KAA8782864.1.
References
1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.