Difference between revisions of "Part:BBa K3408007"

 
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<partinfo>BBa_K3408007 short</partinfo>
 
<partinfo>BBa_K3408007 short</partinfo>
  
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Used PliaG(BBa_K823000), RBS(BBa_B0034), lacI(BBa_C0012), terminator(BBa_B0015), Pgrac(BBa_K1628202), mazF(BBa_K1096002) to avoid expressing mazF when IPTG was added.
+
Used P<sub>nar</sub>(BBa_K3408000), RBS(BBa_B0034), optimized CⅠ(BBa_K3408004), terminator(BBa_B0015), P<sub>CⅠ</sub>(BBa_R0051), RBS(BBa_B0034) and GFP(BBa_E0040) to express GFP when PCⅠ was activated. When our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> was suppressed without FNR and no CⅠ protein could be expressed so that P<sub>CⅠ</sub> could be activated and we could see GFP expressed. But when our <i>Bacillus subtilis</i> was in an anerobic environment, CⅠ protein could be expressed to suppress P<sub>CⅠ</sub> and we couldn't see GFP anymore.
  
 
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Revision as of 12:04, 8 October 2020


Pnar-B0034-phy(ycD)-B0015

Used Pnar(BBa_K3408000), RBS(BBa_B0034), optimized CⅠ(BBa_K3408004), terminator(BBa_B0015), PCⅠ(BBa_R0051), RBS(BBa_B0034) and GFP(BBa_E0040) to express GFP when PCⅠ was activated. When our Bacillus subtilis was in an aerobic environment, Pnar was suppressed without FNR and no CⅠ protein could be expressed so that PCⅠ could be activated and we could see GFP expressed. But when our Bacillus subtilis was in an anerobic environment, CⅠ protein could be expressed to suppress PCⅠ and we couldn't see GFP anymore.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]