Difference between revisions of "Part:BBa K3408000"
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| − | We add the promoter “P<sub>nar</sub>nar”, <b>in response to the RES-FNR oxygen regulator system</b> of <i>Bacillus Subtilis</i>, to express <b>phy(ycD) phytase</b> efficiently in the absence of oxygen, and meanwhile express <b> | + | We add the promoter “P<sub>nar</sub>nar”, <b>in response to the RES-FNR oxygen regulator system</b> of <i>Bacillus Subtilis</i>, to express <b>phy(ycD) phytase</b> efficiently in the absence of oxygen, and meanwhile express <b>CI repressor protein</b> to inhibit toehold switch-Dpnc downstream. |
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According to the paper, they have designed three kinds of strength of the promoter P<sub>nar</sub>nar and applied them in <i>E.coli</i> successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter. | According to the paper, they have designed three kinds of strength of the promoter P<sub>nar</sub>nar and applied them in <i>E.coli</i> successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter. | ||
Revision as of 13:55, 5 October 2020
We add the promoter “Pnarnar”, in response to the RES-FNR oxygen regulator system of Bacillus Subtilis, to express phy(ycD) phytase efficiently in the absence of oxygen, and meanwhile express CI repressor protein to inhibit toehold switch-Dpnc downstream.
According to the paper, they have designed three kinds of strength of the promoter Pnarnar and applied them in E.coli successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.