Difference between revisions of "Part:BBa K3610038"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | ====BAK1==== | ||
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+ | The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function. | ||
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+ | Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms. | ||
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+ | ====Usage with split-NanoLuc==== | ||
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Revision as of 07:38, 15 October 2020
BAK1 ectodomain / LargeBit for S. cerevisiae
tbd
Usage and Biology
BAK1
The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.
Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.
Usage with split-NanoLuc
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 848
Illegal PstI site found at 893 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 848
Illegal PstI site found at 893 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1278
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 848
Illegal PstI site found at 893 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 848
Illegal PstI site found at 893 - 1000COMPATIBLE WITH RFC[1000]