Difference between revisions of "Part:BBa K3610034"

 
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This part contains the sequence for the ectodomain of the plant surface receptor BAK1 fused to the N-terminal part of a split-mCherry protein. Additionally, instead of the signal peptide native to the plant receptor, there is the secretion signal of the alpha factor from yeast at the N-terminal domain of the receptor, replacing the original signal sequence.
  
 
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===Usage and Biology===
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===Biology===
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====BAK1====
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The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.
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Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.
  
 
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Revision as of 15:02, 14 October 2020


BAK1 ectodomain / mCherry N-terminal

This part contains the sequence for the ectodomain of the plant surface receptor BAK1 fused to the N-terminal part of a split-mCherry protein. Additionally, instead of the signal peptide native to the plant receptor, there is the secretion signal of the alpha factor from yeast at the N-terminal domain of the receptor, replacing the original signal sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 1000
    COMPATIBLE WITH RFC[1000]