Difference between revisions of "Part:BBa K3453024:Design"

 
 
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===Design Notes===
 
===Design Notes===
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to do
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designed using our internal toehold switch pipeline which calls NUPACK [3] and RBS Calculator for switch prediction and ranking [4]. For full details, visit [https://2020.igem.org/Team:Evry_Paris-Saclay/Model the 'Model' page of our wiki].
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+
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===Source===
 
===Source===
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===References===
 
===References===
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[1] Hassold S, Lowry PP 2nd, Bauert MR, Razafintsalama A, Ramamonjisoa L, Widmer A. DNA barcoding of Malagasy rosewoods: towards a molecular identification of CITES-listed ''Dalbergia'' species. PLoS One (2016) 11, e0157881.
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 +
[2] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
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 +
[3] Zadeh JN, Steenberg CD, Bois JS, Wolfe BR, Pierce MB, Khan AR, Dirks RM, Pierce NA. NUPACK: Analysis and design of nucleic acid systems. Journal of Computational Chemistry (2011) 32, 170–173.
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 +
[4] Salis HM. The ribosome binding site calculator. Methods in Enzymology (2011) 498, 19–42.

Latest revision as of 22:32, 26 October 2020


DmMatK Trigger 2.1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 26
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 26
    Illegal BamHI site found at 37
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

designed using our internal toehold switch pipeline which calls NUPACK [3] and RBS Calculator for switch prediction and ranking [4]. For full details, visit the 'Model' page of our wiki.

Source

DNA synthesis

References

[1] Hassold S, Lowry PP 2nd, Bauert MR, Razafintsalama A, Ramamonjisoa L, Widmer A. DNA barcoding of Malagasy rosewoods: towards a molecular identification of CITES-listed Dalbergia species. PLoS One (2016) 11, e0157881.

[2] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

[3] Zadeh JN, Steenberg CD, Bois JS, Wolfe BR, Pierce MB, Khan AR, Dirks RM, Pierce NA. NUPACK: Analysis and design of nucleic acid systems. Journal of Computational Chemistry (2011) 32, 170–173.

[4] Salis HM. The ribosome binding site calculator. Methods in Enzymology (2011) 498, 19–42.