Difference between revisions of "Part:BBa K3328010:Design"

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===Design Notes===
 
===Design Notes===
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https://2020.igem.org/wiki/images/thumb/b/b9/T--OUC-China--design_fig2.png/800px-T--OUC-China--design_fig2.png.jpeg
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Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation.
  
 
===Source===
 
===Source===

Revision as of 09:17, 27 October 2020


trigger of toehold


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

800px-T--OUC-China--design_fig2.png.jpeg

Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation.

Source

synthesize from company

References