Difference between revisions of "Part:BBa K123003"

 
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<partinfo>BBa_K123003 short</partinfo>
 
<partinfo>BBa_K123003 short</partinfo>
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K123003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K123003 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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[[image:toxicity]]
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'''Figure 1:'''<br>
  
  

Revision as of 03:14, 29 October 2008

ER

This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E.Coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In Humans this estrogen receptor (ER) is a transcription factor that inciates transcription. However because we are interested in using this protien in bacteria we could not assume the same enhancer like effects. We have designed this part to act as a repressor when bound to the ERE by physically blocking the Polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 24
    Illegal NgoMIV site found at 902
    Illegal AgeI site found at 870
    Illegal AgeI site found at 1841
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

File:Toxicity Figure 1: