Difference between revisions of "Part:BBa K103016"

Usage and Biology

• It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit omega ( BBa_K103012)
• Fusion partners are connected via Gly-Ser-Gly linker
• It's base for creation of hunter fusions in 'hunter and prey' selection system
• Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
• Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
• This part uses our NdeI/SacI/BamHI fusion cloning substandard

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.