Difference between revisions of "Part:BBa K3570008"
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<partinfo>BBa_K3570008 short</partinfo> | <partinfo>BBa_K3570008 short</partinfo> | ||
+ | <h2>Usage</h2> | ||
The HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. | The HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. | ||
When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid. | When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid. | ||
− | + | <h2>Source</h2> | |
− | + | ||
− | 2- https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3 | + | *[1]GenBank: U03439.1 |
+ | *[2]RS313 plasmid https://www.addgene.org/vector-database/3972/ | ||
+ | *[3]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3 | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 18:39, 12 October 2020
HIS3 selection marker
Usage
The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.
When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.
Source
- [1]GenBank: U03439.1
- [2]RS313 plasmid https://www.addgene.org/vector-database/3972/
- [3]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1005
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 896
Illegal BglII site found at 956 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]