Difference between revisions of "Part:BBa K3408000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The spacer sequence of 15 bp between the - 35 and - 10 elements of the upstream region of the wild-type nar promoter was randomized. | + | The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized. |
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===Source=== | ===Source=== | ||
Revision as of 01:06, 28 October 2020
promoter nar(fnr regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized.
Source
The sequence is found through the paper <Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli>.
References
Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli[J]. Applied and Environmental Biology, 2014, 20(02):295-299.