Difference between revisions of "Part:BBa K3570016"
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<partinfo>BBa_K3570016 short</partinfo> | <partinfo>BBa_K3570016 short</partinfo> | ||
− | + | YPRCtau3 upstream homologous arm used together with YPRCtau3 downstream (BBa_K3570017). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI. | |
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Revision as of 00:15, 3 October 2020
YPRCtau3 upstream homologous sequence
YPRCtau3 upstream homologous arm used together with YPRCtau3 downstream (BBa_K3570017). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 137
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 137
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 524
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 137
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 137
- 1000COMPATIBLE WITH RFC[1000]