Difference between revisions of "Part:BBa K3570013"
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<partinfo>BBa_K3570013 short</partinfo> | <partinfo>BBa_K3570013 short</partinfo> | ||
| − | The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP) | + | The HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP) |
When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid. | When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid. | ||
| + | |||
| + | Source: | ||
| + | Taken from FL38 plasmid (https://www.addgene.org/vector-database/2750/) | ||
| + | |||
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Revision as of 18:01, 3 October 2020
URA3 selection marker
The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP)
When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.
Source: Taken from FL38 plasmid (https://www.addgene.org/vector-database/2750/)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 278
Illegal BglII site found at 1374 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]