Difference between revisions of "Part:BBa K3570010"
Line 3: | Line 3: | ||
<partinfo>BBa_K3570010 short</partinfo> | <partinfo>BBa_K3570010 short</partinfo> | ||
− | HO downstream | + | <h2>Usage</h2> |
+ | HO downstream homology arm part used together with HO upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570009 BBa_K3570009]). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene. | ||
+ | |||
+ | <h2>Experiments</h2> | ||
+ | |||
+ | <h2>Source</h2> | ||
+ | |||
+ | *[1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1 | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 04:48, 13 October 2020
HO downstream homologous sequence
Usage
HO downstream homology arm part used together with HO upstream homology arm part (BBa_K3570009). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
Experiments
Source
- [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]