Difference between revisions of "Part:BBa K3406005"

 
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<partinfo>BBa_K3406005 short</partinfo>
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CcaCas13b is a member of Cas13 protein family.It has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be actived by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA.
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Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly U/UA/UC
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3406005 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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===Cloning,expression and purification===
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===References===
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Revision as of 11:47, 1 October 2020

6xHis/Twin-strep-SUMO-CcaCas13b CcaCas13b is a member of Cas13 protein family.It has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be actived by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA. Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly U/UA/UC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1436
    Illegal AgeI site found at 2288
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

waiting to be edited...

Cloning,expression and purification

References

waiting to be edited...