Difference between revisions of "Part:BBa K3610001"
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<partinfo>BBa_K3610001 short</partinfo>
 
<partinfo>BBa_K3610001 short</partinfo>
  
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This part contains the cDNA of the full-length plant cell surface receptor BAK1 from A. thaliana.
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This part contains the cDNA of the full-length plant cell surface receptor BAK1 from <i>A. thaliana</i>.
  
 
===Usage and Biology===
 
===Usage and Biology===
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In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence.
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In our project we used this receptor to demonstrate expression of plant PRRs in <i>S. cerevisiae</i> by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence.
 
For characterization of the part see [[Part:BBa_K3610030]]
 
For characterization of the part see [[Part:BBa_K3610030]]
  

Revision as of 16:49, 19 October 2020


BAK1 from Arabidopsis thaliana

This part contains the cDNA of the full-length plant cell surface receptor BAK1 from A. thaliana.

Usage and Biology

The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.

Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.


In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence. For characterization of the part see Part:BBa_K3610030


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1560
    Illegal PstI site found at 650
    Illegal PstI site found at 695
    Illegal PstI site found at 985
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1560
    Illegal PstI site found at 650
    Illegal PstI site found at 695
    Illegal PstI site found at 985
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1560
    Illegal PstI site found at 650
    Illegal PstI site found at 695
    Illegal PstI site found at 985
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1560
    Illegal PstI site found at 650
    Illegal PstI site found at 695
    Illegal PstI site found at 985
  • 1000
    COMPATIBLE WITH RFC[1000]