Difference between revisions of "Part:BBa K3349006"
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<partinfo>BBa_K3349006 short</partinfo> | <partinfo>BBa_K3349006 short</partinfo> | ||
| − | This Pectin Lyase is from the organism <i>Paenibacillus amylolyticus</i>. | + | This Pectin Lyase is from the organism <i> Paenibacillus amylolyticus </i>. Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. A hexa-histidine tag was added to the C-terminus of the coding sequence for nickel affinity purification. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS |
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| + | For building thermostable variants of this Pnl enzyme we first did homology modelling as the crystal structure has not been determined. | ||
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| + | <img src= "https://2020.igem.org/wiki/images/c/c0/T--Lethbridge_HS--Pnl_modelling.png" alt="pnl modelling" style="width:700px;height:300px;"> | ||
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| + | </html> | ||
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| + | <b>Figure 1:</b> Homology modelling of Pnl from <i>P. amylolyticus </i>. The protein structure was done using iTASSER [2] and SWISS-Model [3]. The two structures were then aligned and compared. A more in depth analysis can be found on our webpage. | ||
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Revision as of 17:07, 29 September 2020
Pectin Lyase Pnl
This Pectin Lyase is from the organism Paenibacillus amylolyticus . Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. A hexa-histidine tag was added to the C-terminus of the coding sequence for nickel affinity purification. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS
For building thermostable variants of this Pnl enzyme we first did homology modelling as the crystal structure has not been determined.
Figure 1: Homology modelling of Pnl from P. amylolyticus . The protein structure was done using iTASSER [2] and SWISS-Model [3]. The two structures were then aligned and compared. A more in depth analysis can be found on our webpage.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]