Difference between revisions of "Part:BBa K3349003"

Revision as of 15:55, 6 October 2020

Pectin Lyase Pnl-GFP

This Pectin lyase is originally from Paenabacillus amylolyticus . Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. Keggi and Doran-Peterson found this pectinase had a Km of 0.13 mg/ml and a Vmax of 144.6+/- 1.894 iU/mg when a 80% methylated substrate was used. Optimal reaction temperature was found to be 55°C and between a pH of 9-10 [1]. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or more analysis of the pnl gene see part BBa_K3349000.

A GFP reporter has been fused via a GC linker to the C terminal end of the protein. As well a C-terminal his tag was also added.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3349003 short</partinfo>
-This Pectin lyase is originally from <i> Paenabacillus amylolyticus </i>. A GFP reporter has been fused via a GC linker to the C terminal end of the protein.
+This Pectin lyase is originally from <i> Paenabacillus amylolyticus </i>. Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. Keggi and Doran-Peterson found this pectinase had a Km of 0.13 mg/ml and a Vmax of 144.6+/- 1.894 iU/mg when a 80% methylated substrate was used. Optimal reaction temperature was found to be 55°C and between a pH of 9-10 [1]. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or more analysis of the pnl gene see part BBa_K3349000.
+A GFP reporter has been fused via a GC linker to the C terminal end of the protein. As well a C-terminal his tag was also added.
 <!-- Add more about the biology of this part here