Difference between revisions of "Part:BBa K3498004"
(AbrB protein inhibits B. subtilis sporulation) |
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− | AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of | + | |
− | numerous that is present in vegetatively growing cells and is inactivated or eliminated by | + | __NOTOC__ |
+ | <partinfo>BBa_K3317007 short</partinfo> | ||
+ | |||
+ | Sensitive promoter against protocatechuic acid and presents its maximum induction in the presence of 1000mM RPU 1.6 of vanillinic acid with a leak of 4.5x10-3 RPU in absence of induction(Meyer, A. J <i> et. al</i> 2019) this part needs its regulatory protein (<partinfo>K3317005 </partinfo>) | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3317007 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous that is present in vegetatively growing cells and is inactivated or eliminated by | ||
the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding | the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding | ||
protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm | protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm | ||
and sporulation formation in B. subtilis, it affects the production by binding into DNA and | and sporulation formation in B. subtilis, it affects the production by binding into DNA and | ||
blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017). | blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017). |
Revision as of 05:40, 22 September 2020
Inducible p3B5C promoter
Sensitive promoter against protocatechuic acid and presents its maximum induction in the presence of 1000mM RPU 1.6 of vanillinic acid with a leak of 4.5x10-3 RPU in absence of induction(Meyer, A. J et. al 2019) this part needs its regulatory protein (BBa_K3317005)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous that is present in vegetatively growing cells and is inactivated or eliminated by
the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding
protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm
and sporulation formation in B. subtilis, it affects the production by binding into DNA and
blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017).