Difference between revisions of "Part:BBa K3351001"

 
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<partinfo>BBa_K3351001 short</partinfo>
 
<partinfo>BBa_K3351001 short</partinfo>
  
It is a ribosome binding site from pet28a plasmid.
+
The ribosome binding site (RBS), defined as the region within the mRNA that is protected by the bound 30S subunit in the initiation complex, was reported to extend from positions –18 to +10 relative to the start codon. This RBS in plasmid PET28a is suitable for general protein expression in E. coli. Stable structures within an RBS should impair binding and interfere with initiation. Its local structure rather than the stability of an SD-antiSD interaction predicts output. So the accessibility of an RBS affects protein output.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:58, 19 October 2020


A ribosome binding site from pet28a plasmid.

The ribosome binding site (RBS), defined as the region within the mRNA that is protected by the bound 30S subunit in the initiation complex, was reported to extend from positions –18 to +10 relative to the start codon. This RBS in plasmid PET28a is suitable for general protein expression in E. coli. Stable structures within an RBS should impair binding and interfere with initiation. Its local structure rather than the stability of an SD-antiSD interaction predicts output. So the accessibility of an RBS affects protein output.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]