Difference between revisions of "Part:BBa K143078"
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<partinfo>BBa_K143078 short</partinfo> | <partinfo>BBa_K143078 short</partinfo> | ||
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GFP gene under expresson of the P43 promoter and spoVG RBS of ''B. subtilis'', with a chloramphenicol resistance marker for ease of selection. | GFP gene under expresson of the P43 promoter and spoVG RBS of ''B. subtilis'', with a chloramphenicol resistance marker for ease of selection. | ||
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Also contains the 5' and 3' amyE integration sequences to allow integration into ''B. subtilis'' at the amyE locus. | Also contains the 5' and 3' amyE integration sequences to allow integration into ''B. subtilis'' at the amyE locus. | ||
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This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part <bbpart>BBa_K143051</bbpart>) as part of the Biofabricator ''subtilis'' project. | This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part <bbpart>BBa_K143051</bbpart>) as part of the Biofabricator ''subtilis'' project. | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
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<partinfo>BBa_K143078 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143078 SequenceAndFeatures</partinfo> | ||
Latest revision as of 18:45, 27 October 2008
P43-spoVG GFP expression construct
GFP gene under expresson of the P43 promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.
Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.
This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part BBa_K143051) as part of the Biofabricator subtilis project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2143