Difference between revisions of "Part:BBa K3447000:Design"

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===Design Notes===
 
===Design Notes===
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abaaba
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There is a repetitive sequence between <i>cvi</i> and <i>cvaC</i> gene, and other than that there is no need to pay attention.
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===Source===
 
===Source===

Revision as of 14:29, 4 September 2020


luxI, an autoinducer synthetase for AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a repetitive sequence between cvi and cvaC gene, and other than that there is no need to pay attention.

Source

We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.

References