Difference between revisions of "Part:BBa K3447000:Design"

(Source)
(Source)
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===Source===
 
===Source===
  
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
+
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.<br>
 +
This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
 +
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075<br>
 +
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164<br>
 +
We ordered the part DNA from a synthesis company.
  
 
===References===
 
===References===

Revision as of 14:26, 4 September 2020


luxI, an autoinducer synthetase for AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

abaaba


Source

We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.

References