Difference between revisions of "Part:BBa K3610036"

Revision as of 13:22, 5 October 2020

BAK1 ectodomain / mCherry N-terminal - for C. reinhardtii

This part is the cell surface receptor BAK1 from A. thaliana fused with the N-terminal sequence of a split-mCherry protein. The two protein coding sequences are fused together with a 15 amino acid linker, coding for glycin and serine. As a promoter a truncated version of the ADH1 version was used as it confers lower expression levels. To mediate termination of transcription the ScENO2 terminator from S. cerevisiae was used.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 93
Illegal NheI site found at 925
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 855
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3610036 short</partinfo>
-This part is the cell surface receptor BAK1 from A. thaliana fused with a GFP. The two protein coding sequences are fused together with a 15 amino acid linker, coding for glycin and serine.
+This part is the cell surface receptor BAK1 from A. thaliana fused with the N-terminal sequence of a split-mCherry protein. The two protein coding sequences are fused together with a 15 amino acid linker, coding for glycin and serine.
 As a promoter a truncated version of the ADH1 version was used as it confers lower expression levels.
 To mediate termination of transcription the ScENO2 terminator from S. cerevisiae was used.
-BAK1/GFP is designed for the expression of a the full length plant receptor BAK1 from A. thaliana together with a fluorescent GFP tag in yeast. This allows checking for the localization and the level of expression of the BAK1 protein in the yeast cell. Being the full length cell surface protein, the BAK1 sequence has its own signal sequence from the orignal organism.
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