Difference between revisions of "Part:BBa K3610001"

Revision as of 18:11, 30 September 2020

BAK1 from Arabidopsis thaliana

This part contains the cDNA of the full-length plant cell surface receptor BAK1 from A. thaliana.

Usage and Biology

The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.

Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.

In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence. For characterization of the part see Part:BBa_K3610030

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3610001 short</partinfo>
+This part contains the cDNA of the full-length plant cell surface receptor BAK1 from A. thaliana.
+===Usage and Biology===
 The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome.
 The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.
-Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR),  FLS2 and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.
+Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.
-<!-- Add more about the biology of this part here
+In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence.
-===Usage and Biology===
+For characterization of the part see [[Part:BBa_K3610030]]
 <!-- -->