Difference between revisions of "Part:BBa K3458003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | *The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of ''Oryza sativa L.''. The promoter GluD-1 ([https://parts.igem.org/Part:BBa_K3458002 BBa_K3458002]) that we plan to use with specific expression characteristics in ''Oryza sativa L.'' endosperm cannot drive HQT express in rice protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT composite part for comparison with the GluD-1 promoter + HQT part ([https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004]) . | |
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===Source=== | ===Source=== | ||
Revision as of 07:47, 17 August 2020
35S Promoter + HQT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 191
Design Notes
- The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of Oryza sativa L.. The promoter GluD-1 (BBa_K3458002) that we plan to use with specific expression characteristics in Oryza sativa L. endosperm cannot drive HQT express in rice protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT composite part for comparison with the GluD-1 promoter + HQT part (BBa_K3458004) .
Source
A