Difference between revisions of "Part:BBa J119450"

Latest revision as of 19:24, 1 July 2020

R Clone of Schistosome Ribozyme

The R Clone of Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a four nucleotide (nt) base change from the Schistosome Ribozyme (J119447) in two locations. At nt 18-21, the original ribozyme sequence of GGGC was edited to CCCC. At nt 61-64, the original ribozyme sequence of GAGA was edited to GGGG. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for this ribozyme is found in Computational Design and Biosensor Application of Small Molecule-Sensing Allosteric Ribozymes (https://pubs.acs.org/doi/abs/10.1021/bm400299a).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 19:16, 1 July 2020 (view source) Registry (Talk \| contribs)		Latest revision as of 19:24, 1 July 2020 (view source) Eckdahl (Talk \| contribs)
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	<partinfo>BBa_J119450 short</partinfo>		<partinfo>BBa_J119450 short</partinfo>

−	The R Clone of Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a four nucleotide (nt) base change from the Schistosome Ribozyme (J119447) in two locations. At nt 44-47, the original ribozyme sequence of GGGC was edited to CCCC. At nt 87-90, the original ribozyme sequence of GAGA was edited to GGGG. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for this ribozyme is found in Computational Design and Biosensor Application of Small Molecule-Sensing Allosteric Ribozymes (https://pubs.acs.org/doi/abs/10.1021/bm400299a).	+	The R Clone of Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a four nucleotide (nt) base change from the Schistosome Ribozyme (J119447) in two locations. At nt 18-21, the original ribozyme sequence of GGGC was edited to CCCC. At nt 61-64, the original ribozyme sequence of GAGA was edited to GGGG. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for this ribozyme is found in Computational Design and Biosensor Application of Small Molecule-Sensing Allosteric Ribozymes (https://pubs.acs.org/doi/abs/10.1021/bm400299a).