Difference between revisions of "Part:BBa J119446"
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| − | The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in | + | The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y). |
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Latest revision as of 18:55, 18 June 2020
Mammal Ribozyme
The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]