Difference between revisions of "Template:TypeIIS/Plasmids/Level1"
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| − | + | Level 1 transcriptional units in this set of plasmid backbones will be flanked by SapI recognition sites and fusion sites, enabling further assembly of up to 4 transcriptional units as a Level 2 Assembly. | |
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| − | '''Note:''' Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert <partinfo>BBa_J04454</partinfo> have been verified. | + | |
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| + | <center> | ||
| + | [[File:TypeIIS-Level1-J04454-Simple.png]]<br> | ||
| + | '''Simplified diagram of BBa_J04454 and pSB1K0# with BsaI cut site.''' | ||
| + | </center> | ||
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| + | When four Level 0 parts are assembled into the following plasmid backbones (with <partinfo>BBa_J04454</partinfo>), the resulting transcriptional unit (TU) will be flanked by SapI restriction sites and these 3 bp Fusion Sites (NNN), which can be used for subsequent (Level 2) assemblies. | ||
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| + | <center>[[File:TypeIIS-Level1-L0-Part-Assembly-Simple.png|900px]]</center> | ||
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| + | <center>[[File:TypeIIS-Level1-TU-Simple.png]]</center> | ||
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| + | '''Note:''' | ||
| + | *Since <partinfo>pSB1K3</partinfo> is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies. | ||
| + | *Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert <partinfo>BBa_J04454</partinfo> have been verified. | ||
Revision as of 20:15, 17 June 2020
Level 1 transcriptional units in this set of plasmid backbones will be flanked by SapI recognition sites and fusion sites, enabling further assembly of up to 4 transcriptional units as a Level 2 Assembly.
| Registry Name | Loop Name | Fusion Site 5' | TU | Fusion Site 3' |
|---|---|---|---|---|
| pSB1K01 | pOdd1 | ATG | TU 1 | GCA |
| pSB1K02 | pOdd2 | GCA | TU 2 | TAC |
| pSB1K03 | pOdd3 | TAC | TU 3 | CAG |
| pSB1K04 | pOdd4 | CAG | TU 4 | GGT |
Simplified diagram of BBa_J04454 and pSB1K0# with BsaI cut site.
When four Level 0 parts are assembled into the following plasmid backbones (with BBa_J04454), the resulting transcriptional unit (TU) will be flanked by SapI restriction sites and these 3 bp Fusion Sites (NNN), which can be used for subsequent (Level 2) assemblies.
Note:
- Since pSB1K3 is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies.
- Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04454 have been verified.