Difference between revisions of "Template:TypeIIS/Plasmids/Level1"
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When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with <partinfo>BBa_J04454</partinfo>), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites. | When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with <partinfo>BBa_J04454</partinfo>), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites. | ||
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| + | Since <partinfo>pSB1K3</partinfo> is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies. | ||
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Revision as of 20:00, 15 June 2020
When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with BBa_J04454), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.
Since pSB1K3 is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies.
| Registry Name | Loop Name | Fusion Site 5' | TU | Fusion Site 3' |
|---|---|---|---|---|
| pSB1K01 | pOdd1 | ATG | TU 1 | GCA |
| pSB1K02 | pOdd2 | GCA | TU 2 | TAC |
| pSB1K03 | pOdd3 | TAC | TU 3 | CAG |
| pSB1K04 | pOdd4 | CAG | TU 4 | GGT |