Difference between revisions of "Template:TypeIIS/Plasmids/Level1"

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</tr>
 
</tr>
 
<tr>
 
<tr>
<td></html><partinfo>pSB1K02</partinfo><html></td>
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<td></html>[[Part:pSB1K02|pSB1K02]]<html></td>
 
<td>pOdd2</td>
 
<td>pOdd2</td>
 
<td class="dnaSeq rightAlign">GCA</td>
 
<td class="dnaSeq rightAlign">GCA</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
<td></html><partinfo>pSB1K03</partinfo><html></td>
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<td></html>[[Part:pSB1K03|pSB1K03]]<html></td>
 
<td>pOdd3</td>
 
<td>pOdd3</td>
 
<td class="dnaSeq rightAlign">TAC</td>
 
<td class="dnaSeq rightAlign">TAC</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
<td></html><partinfo>pSB1K04</partinfo><html></td>
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<td></html>[[Part:pSB1K04|pSB1K04]]<html></td>
 
<td>pOdd4</td>
 
<td>pOdd4</td>
 
<td class="dnaSeq rightAlign">CAG</td>
 
<td class="dnaSeq rightAlign">CAG</td>

Revision as of 19:52, 15 June 2020

When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with BBa_J04454), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.

Registry Name Loop Name Fusion Site 5' TU Fusion Site 3'
pSB1K01 pOdd1 ATG TU 1 GCA
pSB1K02 pOdd2 GCA TU 2 TAC
pSB1K03 pOdd3 TAC TU 3 CAG
pSB1K04 pOdd4 CAG TU 4 GGT
Note: Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04454 have been verified.