Difference between revisions of "Part:BBa K3600000"
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* activators: MSN2/4 | * activators: MSN2/4 | ||
*HSP12 gene encodes Saccharomyces Cerevisiae's small heat shock proteins.<html><a href="#varela1995"><sup>1</sup></a></html> | *HSP12 gene encodes Saccharomyces Cerevisiae's small heat shock proteins.<html><a href="#varela1995"><sup>1</sup></a></html> | ||
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*The promoter HSP12 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to a stress. | *The promoter HSP12 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to a stress. | ||
| + | *The yeast toolkit for modular assembly (ref) was used to assemble transcriptional units. This promoter was used as a part of type II. | ||
*The induction of gene transcription is mediated by stress response elements STRE.<html><a href="#Martínez-Pastor1996"><sup>2</sup></a></html> | *The induction of gene transcription is mediated by stress response elements STRE.<html><a href="#Martínez-Pastor1996"><sup>2</sup></a></html> | ||
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<partinfo>BBa_K3600000 parameters</partinfo> | <partinfo>BBa_K3600000 parameters</partinfo> | ||
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===Measurements=== | ===Measurements=== | ||
Revision as of 17:55, 14 June 2020
HSP12 promoter part plasmid
This part contains the promoter of HSP12, a heat-shock protein in Saccharomyces Cerevisiae that is activated by the high-osmolarity glycerol (HOG) pathway.1
Usage and Biology
- activators: MSN2/4
- HSP12 gene encodes Saccharomyces Cerevisiae's small heat shock proteins.1
- The promoter HSP12 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to a stress.
- The yeast toolkit for modular assembly (ref) was used to assemble transcriptional units. This promoter was used as a part of type II.
- The induction of gene transcription is mediated by stress response elements STRE.2
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 308
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 326
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 308
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 308
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 57
Illegal BsaI.rc site found at 578